ids 171124 171127 Search Results


addgene ids 171124 171127  (Addgene inc)
93
Addgene inc addgene ids 171124 171127
Addgene Ids 171124 171127, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/addgene ids 171124 171127/product/Addgene inc
Average 93 stars, based on 1 article reviews
addgene ids 171124 171127 - by Bioz Stars, 2026-05
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plasmid pdb4581  (Addgene inc)
90
Addgene inc plasmid pdb4581
The plasmids created and used in this study
Plasmid Pdb4581, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid pdb4581/product/Addgene inc
Average 90 stars, based on 1 article reviews
plasmid pdb4581 - by Bioz Stars, 2026-05
90/100 stars
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The plasmids created and used in this study

Journal: G3: Genes|Genomes|Genetics

Article Title: An improved auxin-inducible degron system for fission yeast

doi: 10.1093/g3journal/jkab393

Figure Lengend Snippet: The plasmids created and used in this study

Article Snippet: Primers used for plasmid construction are listed in Supplementary Table S1. pDB4581, pDB4697, pDB5050, and pDB4695 have been deposited at Addgene (Addgene IDs 171124–171127) and the Yeast Genetic Resource Center of Japan (YGRC/NBRP) ( https://yeast.nig.ac.jp, last accessed November 19, 2021 ) (NBRP IDs FYP4960–4963).

Techniques: Plasmid Preparation, Control, Expressing

3×sAID is a more effective degron than the full-length IAA17. (A) Schematic of the 3×sAID degron in the tagging plasmid pDB4581. (B) Schematic of endogenous C-terminal tagging of a gene with the 3×sAID degron in the plasmid pDB4581 (not drawn to scale). ADH1ter and kanMX are the terminator of S. cerevisiae ADH1 gene and the G418-resistance selection marker, respectively. (C) Spot assay showed that growth inhibition occurred at a lower concentration of NAA when the Padh1-OsTIR1 cassette was combined with mcm4-3×sAID than when combined with mcm4-IAA17 .

Journal: G3: Genes|Genomes|Genetics

Article Title: An improved auxin-inducible degron system for fission yeast

doi: 10.1093/g3journal/jkab393

Figure Lengend Snippet: 3×sAID is a more effective degron than the full-length IAA17. (A) Schematic of the 3×sAID degron in the tagging plasmid pDB4581. (B) Schematic of endogenous C-terminal tagging of a gene with the 3×sAID degron in the plasmid pDB4581 (not drawn to scale). ADH1ter and kanMX are the terminator of S. cerevisiae ADH1 gene and the G418-resistance selection marker, respectively. (C) Spot assay showed that growth inhibition occurred at a lower concentration of NAA when the Padh1-OsTIR1 cassette was combined with mcm4-3×sAID than when combined with mcm4-IAA17 .

Article Snippet: Primers used for plasmid construction are listed in Supplementary Table S1. pDB4581, pDB4697, pDB5050, and pDB4695 have been deposited at Addgene (Addgene IDs 171124–171127) and the Yeast Genetic Resource Center of Japan (YGRC/NBRP) ( https://yeast.nig.ac.jp, last accessed November 19, 2021 ) (NBRP IDs FYP4960–4963).

Techniques: Plasmid Preparation, Selection, Marker, Spot Test, Inhibition, Concentration Assay